Composite

Part:BBa_K5048071:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


T7 promoter-Neae-LAP-6xHis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
    Illegal PstI site found at 3613
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3613
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4656
    Illegal BglII site found at 4754
    Illegal BamHI site found at 92
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
    Illegal PstI site found at 3613
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
    Illegal PstI site found at 3613
    Illegal NgoMIV site found at 2862
    Illegal NgoMIV site found at 4128
    Illegal AgeI site found at 2494
    Illegal AgeI site found at 4150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1344
    Illegal SapI site found at 4476


Design Notes

We constructed this part by restriction endonuclease cleavage and T4 enzyme ligation. Due to the multiple cleavage sites on the LAP gene, we had to choose enzymes meticulously.


Source

See our parts:BBa_I719005, BBa_K5048047,BBa_K5048058


References